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The MED64 planar microelectrode array recording system is a complete and easy-to-use multi-channel isolated extracellular neuroelectrophysiological research tool produced by Alpha Med Science, Japan. The system includes a complete set of hardware equipment consumables and analysis software required for in vitro electrophysiological studies. The user can conveniently and quickly perform electrophysiological examination on ex vivo materials such as brain slices, myocardial sections, cultured nerve cells or cardiomyocytes, stem cells, etc. The cells or tissues to be cultured directly on the electrode coating material can be allowed to measure. Local electrical changes directly adjacent to the cell, while simultaneously recording the electrophysiological signals of multiple cells. MED64 devices can simultaneously record and stimulate different sites; moreover, MED64 records are non-invasive and therefore do not damage cells. This feature makes them suitable for long-term recording and repeated recording on the same culture sample, thus allowing The longer-term evolution of the response of the culture to drug stimulation was monitored. Combined with the optical recording method, photoelectric combined detection can be realized.
Long-term potentiation of synaptic transmission in the insular cortex of adult mice
Multi-electrode array recording system application
Long-term potentiation of synaptic transmission in the insular cortex of adult mice
Long-term potentiation of synaptic transmission in the adult mouse insular cortex: multi-electrode array recordings
Ming-Gang Liu1, SukJae-Joshua Kang2, Tian-Yao Shi3, Kohei Koga4, Ming-Ming Zhang3, Graham L. Collingridge5, Bong-Kiun Kaang2, and Min Zhuo6,*
Articles in PresS. J Neurophysiol (May 1, 2013). doi:10.1152/jn.01104.2012
The insular cortex (IC) is widely considered to be an important forebrain structure involving cognitive and sensory processes such as memory and pain. However, only a few of the brain function studies of the relevant island cortex are directed at the synaptic basis at the cellular level. This study aimed to understand the long-term potentiation (LTP) synaptic mechanisms of insula. Using a 64-channel extracellular electric field recording system (Med64, AlphaMed, Osaka), we found that after stimulating different layers of IC chips using θ rhythm strings, at least 3 hours of persistent late-onset LTP (L-LTP) were reliably recorded. Insular LTP induction is protein synthesis-dependent, and the process requires activation of the GluN2A GluN2B subunit of the NMDA receptor, L-type voltage-gated calcium channel and metabotropic glutamate receptor 1. The double pulse facilitation effect ratio (PPF ratio) is not affected by the induction of L-LTP of the insula, and the L-LTP expression of the insula leaves requires the presence of a post-synaptic calcium-permeable AMPA receptor. For the first time, our study provides long-term multi-channel extracellular electric field recordings of L-LTP in adult mouse in vitro island cortex, suggesting that it has a potentially important role in memory and chronic pain.
Med64 planar multi-electrode array recording system
original
â— Induced activities (fEPSPs)
â— Spontaneous activity (spiking)
â— Biological rhythm research
â— Synaptic plasticity (LTP/LTD), learning, memory
â— Cardiac research (pacing characteristics and electrical excitation conduction characteristics)
â— Stem cell research
â— Safety pharmacology (such as CNS toxicity and QT interval extension)
â— Acute brain slices for drug testing (epilepsy, Alzheimer's disease, pain, obesity, anorexia, arrhythmia, etc.)
â— Culturing cells or brain slices for chronic drug testing â— QT prolonged screening of iPS / ES cell-derived cardiomyocytes